Read e-book online Density Gradient Centrifugation PDF

By T.S. Work and E. Work (Eds.)

ISBN-10: 0720442214

ISBN-13: 9780720442212

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Extra info for Density Gradient Centrifugation

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These are usually made of synthetic butyl rubber, which has good resistance in most inorganic salts, but not to organic solvents. 0-ringsof Viton (a fluorinated elastomer which is resistant to organic solvents) can be supplied if organic gradients are used. Care should be taken when using CsCl gradients in B-type zonal rotors. Since this salt gives very heavy solutions which are only slightly higher in viscosity than water, blemishes or scratches will allow the caesium chloride to leak out of the rotor.

This cap is usually very reliable, but it should be examined at regular intervals. 8. Guarantees on rotors Although all the manufacturers give what appear to be very comprehensive guarantees, it is worthwhile to compare them and examine them in more detail. We will summarize the guarantees given on swing out and zonal rotors, but the manufacturers must be consulted for the precise conditions. Beckman rotors are guaranteed, subject to conditions, for 1000 runs, o r 2500 hr or 5 years (whichever frst) a t maximum speed.

This problem is overcome in the latest Beckman machines by not mounting the armour bowl rigidly, thus allowing it to spin with the fragments, and so absorb the torque of the blown up rotor. However, there are a number of older machines, whose armour plate may not be strong enough, and one can never be absolutely sure that a blow-up will not cause injury. Besides, a blow-up is likely to produce an expensive repair bill and should be avoided. I h . I. Overspeeding The specified maximum speeds of rotors are designed to give a wide margin of safety.

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Density Gradient Centrifugation by T.S. Work and E. Work (Eds.)


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