By Dawn B. Marks
Temple Univ. institution of medication, Philadelphia, PA. evaluate in define structure for scientific scholars getting ready for USMLE Step 1 or path tests. prior version: c1994. Softcover.
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I 1 3 5 8 ;; 1 3 ' 5 Hours Fig. 10 (continued). at 60°C within a few days or few hours. 3) are extensively destroyed in alkaline solutions (pH 9), acidic pH values (pH 3 to 4) ensuring maximum stability for all Immobiline chemicals (it would thus appear that all these chemicals are most stable in their protonated form). For medium-term storage of precast gels, wet matrices exhibit least degradation if titrated to ca. pH 4, formic acid being the best cn. 1 THE CHEMICALS 31 titrant because it can be efficiently removed during the focusing step with formation of the narrowest salt front at the anode (in addition, in its protonated form it is quite volatile).
13. Control of the degree of polymerization of the Immobiline chemicals. Analysis performed by HPLC in a Bio-Gel TSK 30 column. 8 with 20 mM phosphate. 5 Immobiline were injected and eluted at 1 ml/min flow rate. The eluate was monitored at 220 nm and automatically integrated. 5 stored frozen for up to 1 year). , 198%; with permission of Verlag Chemie). teins in solution, are able to bridge them via two unlike binding surfaces (ionic on one side, hydrophobic in the opposite surface; see the model of bridging of two ferritin molecules depicted in Fig.
6. Problems with the Immobiline chemicals Given such a sophisticated and highly reproducible methodology, the first true example of 'pH gradient engineering', it is important to know how to handle the Immobiline chemicals for optimum performance run after run. Curiously, in the first few years after the introduction of the IPG methodology, not much work was devoted to study the shelf life of the Immobiline species. , 1985). Given their structure, it was apparent that these chemicals could degrade through two major pathways: (1) autopolymerization (a simple check for it is that the solution becomes slightly opalescent) or (2) hydrolysis along the amide bond, thus splitting the acrylamide moiety from the buffering group at the other extremity of the molecule (producing free acrylic acid and an amino acid in the case of the acidic Immobilines and free acrylic acid and a diamine in the case of the basic Immobilines).
Board Review Series: Biochemistry by Dawn B. Marks