Read e-book online Bioquimica de Harper 14 Ed Harper's Biochemistry PDF

By Daryl K. Granner, Peter A. Mayes, Robert Murray

ISBN-10: 9684267568

ISBN-13: 9789684267565

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Extra resources for Bioquimica de Harper 14 Ed Harper's Biochemistry

Example text

No posee libertad de rotación alrededor de[ enlace que conecta a los htomos C y N. En consecuencia, los cuatro iitornos mostrados en la figura 5-7yacen en el mismo plano, esto es, son coplanares. Esta semirrigidez tiene consecuencias importantes para órdenes de estructura proteínica superiores al primer nivel. Por el contrario, hay plena libertad de rotacibn alrededor de los enlaces remanentes del esqueleto polipeptidico. En la figura 5-8, que resume estos conceptos, los enlaces que tienen rotación libre estan circundados por flechas y los átomos coplanares se han sombreado.

C E , . - do aspártic o Áci do glutamico ,inina imagina Cisi:eina Glic:ina G lutamina Histiclina Cisinia a -Serin. - encuentra en regiones donde tos pdptidos se doblan en forma aguda. as moldculas de agua de las proteinas que estan en la vecindad inmediata. Es tlpica la presencia de estos aminohcidos en el interior de proteinas citosólicas. Los grupos R con carga, de los aminoácidos básicos y acidicos, estabilizan las conformaciones proteínicas especificaspor intermedio de la formación de enlaces salinos.

La primera fue la introducci6n por Edman, en 1967, de un procedimiento para la separacion e identificación automfitica de residuos ítmino terminales de aminoácidos asi como sus derivados feniltiohidantoinicos. La segunda fue la introducción independiente por Sanger y por Maxm y Gilbert de tkcnicas para la secuenciación rápida del DNA. En la actualidad, la estrategia óptima consiste en utili7ar ambas técnicas de manera simulthnea. Las estructuras primarias de los polipéptidos se determinan por la técnica automatizada de Edman Dado que numerosas protelnas están constituidas por más de una cadena polipeptidica ligada por fuerzas no covalentes o pos puentes disulfuro, es posible que el primer paso sea disociar y separar las cadenas poli- Figura 5-9.

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Bioquimica de Harper 14 Ed Harper's Biochemistry by Daryl K. Granner, Peter A. Mayes, Robert Murray


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