By Sidney Fleischer and Lester Packer (Eds.)
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Additional info for Biomembranes - Part H: Visual Pigments and Purple Membranes - I
EDWARDS The purpose of this article is to describe methods of preparing primary monolayer cultures of mammalian retinal pigment epithelium (PE) and neural retina. Additional methods for culturing these tissues include organ culture of whole eyes of frog embryos, ~ adult tree toad, z mouseP "4 and ratS-7; suspension cultures of neural retinal cells from embryonic chicks'a; cell lines derived from human retinoblastoma~°'H; organ cultures of human 12"13and bovinC 3 PE-choroid complex; explant culture of human '4 and rat x5 PE; and monolayer cultures of embryonic chick neural retina, 8"~6 and PE.
S. Okada, K. Yasuda, M. Hayashi, Y. Hamada, and G. Eguchi, Dev. Biol. 60, 305 (1977).  ISOLATION AND PURIFICATION OF SQUID RHABDOMS 43 scalpel. Extend the incision circumferentially with microdissection scissors and discard the anterior segment, a2 Allow the posterior eyecups to soak for a few minutes until the neural retina can be gently peeled from the eyecup free of adherent pigment epithelium with two pairs of forceps. The use of pigmented rats greatly facilitates the detection of pigment epithelial contamination.
Edwards and R. B. Szamier, Science 197, 1001 (1977). 16 T. S. Okada, Y. Itoh, K. Watanabe, and G. Eguchi, Dev. Biol. 45, 318 (1975). 17 R. D. Cahn, H. G. Coon, and M. B. Cahn, in " M e t h o d s in Developmental Biology" (F. H. Wilt and N. K. ), p. 493. CroweU (Collier), N e w York, 1967. 18 W. B. Jakoby and I. H. , " M e t h o d s in E n z y m o l o g y , " Volume 58. A c a d e m i c Press, N e w York, 1979. 19 j. Paul, "Cell and Tissue C u l t u r e , " 5th ed. C h u r c h i l l - L i v i n g s t o n e , Edinburgh and London, 1975.
Biomembranes - Part H: Visual Pigments and Purple Membranes - I by Sidney Fleischer and Lester Packer (Eds.)