By Yu. M. Torchinsky, T. T. Berezov (auth.), Dr. Timo K. Korpela, Prof. Philipp Christen (eds.)
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Extra resources for Biochemistry of Vitamin B6 : Proceedings of the 7th International Congress on Chemical and Biological Aspects of Vitamin B6 Catalysis, held in Turku, Finland, June 22–26, 1987
We may therefore conclude that neither the prepiece nor the membrane passage is required for the mature enzyme to attain its active conformation. Electron microscopy of bacterial cells that had expressed mAs pAT showed that the heterologous protein accumulated in circumscript ribosome-free spaces. (Experiments with immuno gold method in collaboration with Drs. P. -Y. Qiao, Dept. of Anatomy, University of Zurich). , 1982). This susceptibility toward proteolytic digestion is also evident intracellularly.
J. Biochem. , and Wada, H. (1980) Biochem. Biophys. Res. Commun. 95, 1781-1788. -(1980) Biochem. Biophys. Res. Commun. ~, 1256-1260. BCR/LS 2 Biochemistry of Vitamin B6 (c) 1987 Birkhauser Verlag Basel 35 MOLECULAR CLONING AND IN VIVO EXPRESSION OF THE PRECURSOR TO RAT MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE J. R. , F. J. Rodriguez-Berrocal, J. Gordon, and M. , Kansas City, MO 64110 USA SUMMARY: A full length cDNA corresponding to rat aspartate aminotransferase has been cloned and partially sequenced.
Seguence of pmAspAT cDNA: The DNA sequence of AspAT-4 up to the start of the mature protein and the corresponding translation product is presented in Figure 2. The first possible initiator codon occurs at nucleotide -87. If protein translation starts at this codon, 29 amino acids would precede the mature protein. This is consistent with known mAspAT presequences and the molecular weight of the in vitro 37 -170 -160 TTTTTTTAGGAGCCCGCGCCTC -150 -140 -130 -120 -110 -100 GGTTTCAGCGGACGTTCCCCTGATCTCCGTTCTACCACCATCCACTGCCGTCTTACCAC -90 -80 -70 -60 -50 -40 CCACCATGGCCCTCCTGCACTCCGGTCGCGTCCTCTCCGGGATGGCTGCTGCCTTTCAC MetAlaLeuLeuHisSerGlyArgValLeuSerGlyMetAlaAlaAlaPheHis -30 -20 -10 -1 1 10 20 CCAGGCCTTGCAGCCGCAGCCTCTGCCAGAGCC AGCTCCTGGTGGACCCATGTTGAA ProglyLeuAlaAlaAlaAlaSerAlaArgAla SerSerTrpTrpThrHisValGlu Figure 2: The DNA and corresponding amino acid sequence of rat pmAspAT.
Biochemistry of Vitamin B6 : Proceedings of the 7th International Congress on Chemical and Biological Aspects of Vitamin B6 Catalysis, held in Turku, Finland, June 22–26, 1987 by Yu. M. Torchinsky, T. T. Berezov (auth.), Dr. Timo K. Korpela, Prof. Philipp Christen (eds.)