By Dr Desmond S. T. Nicholl
Des Nicholl provides a brand new, totally revised, and improved version of his well known undergraduate-level textbook. The publication keeps the various positive factors of the unique variation and nonetheless bargains a concise technical advent to the topic of genetic engineering. it really is divided into 3 major sections: uncomplicated molecular biology, equipment of gene manipulation, and smooth purposes of genetic engineering. functions coated within the publication contain genomics, protein engineering, gene remedy, cloning, transgenic animals and vegetation and bioethics. An advent to Genetic Engineering is key analyzing for undergraduate scholars of biotechnology, genetics, molecular biology, and biochemistry.
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Extra resources for An Introduction to Genetic Engineering
2. Types of ends generated by different restriction enzymes. The enzymes are listed in (a), with their recognition sequences and cutting sites shown in (b) and (c), respectively. (d) A schematic representation of the types of ends generated. Fig. 3. Generation of recombinant DNA. DNA fragments from different sources can be joined together if they have cohesive (‘sticky’) ends, as produced by many restriction enzymes. On annealing the complementary regions, the phosphodiester backbone is sealed using DNA ligase.
1. Recognition sequences and cutting sites for some restriction endonucleases Enzyme Recognition sequence Cutting sites Ends BamHI 5’-GGATCC-3’ 5’ EcoRI 5’-GAATTC-3’ Haelll 5’-GGCC-3’ Hpal 5’-GTTAAC-3’ Pstl 5’-CTGCAG-3’ Sau3A 5’-GATC-3’ Smal 5’-CCCGGG-3’ Sstl 5’-GAGCTC-3’ Xmal 5’-CCCGGG-3’ G↓G A T C C C C T A G↑G G↓A A T T C C T T A A↑G G G↓C C C C↑G G G T T↓A A C C A A↑T T G C T G C A↓G G↑A C G T C ↓ GATC C T A G↑ C C C↓G G G G G G↑C C C G A G C T↓C C↑T C G A G C↓C C G G G G G G C C↑C 5’ Blunt Blunt 3’ 5’ Blunt 3’ 5’ Note: The recognition sequences are given in single-strand form, written 5’→3’.
These days, most biological supply companies sell kits that enable puriﬁcation of nucleic acids from a range of sources. The ﬁrst step in any isolation protocol is disruption of the starting material, which may be viral, bacterial, plant or animal. The method used to open cells should be as gentle as possible, preferably utilising enzymatic degradation of cell wall material (if present) and detergent lysis of cell membranes. If more vigorous methods of cell disruption are required (as is the case with some 28 Working with nucleic acids Fig.
An Introduction to Genetic Engineering by Dr Desmond S. T. Nicholl