Affinity labeling - download pdf or read online

By Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek

ISBN-10: 0121819469

ISBN-13: 9780121819460

The significantly acclaimed laboratory typical, Methods in Enzymology, is likely one of the such a lot hugely revered guides within the box of biochemistry. considering 1955, every one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this present day - really a vital ebook for researchers in all fields of lifestyles sciences

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1, 207 (1971). b W. B. Noroa, A. D. Winer, A. J. Glaid, and G. W. Schwert, J. Biol. Chem. 234, 1143 (1959). c E. Racker, V. Klybas, and M. Schramm, J. Biol. Chem. 234, 2510 (1958). d A. L. Fluharty and C. E. Ballou, J. Biol. Chem. 234, 2517 (1958). • D. Northrop and H. G. Wood, J. Biol. Chem. 244, 5810 (1969). s G. H. Reed and S. D. Morgan, Biochemistry 13, 3537 (1974). • K. D. Collins and G. R. Stark, J. Biol. Chem. 246, 6599 (1971). h W. B. Dempsey and E. E. Snell, Biochemistry 2, 1414 (1973).

E. Merrifield, W. M. Jones, and E. C. Gotschlich, Proc. Natl. Acad. Sci. A. 71, 417 (1974). ~0L. J. Fowler and R. A. John, Biochem. J. 130, 569 (1972). 2, R. A. John and P. Fasella, Biochemistry 8, 4477 (1969). = P. Fasella, in "Pyridoxal Catalysis: Enzymes and Model Systems" (E. ), p. 1. Wiley, New York, 1968. 25C. T. Walsh, A. Schonbrunn, and R. H. Abeles, J. Biol. Chem. 246, 6855 (1971). ,/'~-NI-I+~ ~] /Cl cis- 3-Chloroallylamine 2-Chloroallylamine flavin-linked monoamine oxidase and 2-chloroallylamine is an irreversible inhibit~r and nonflavin-linked monoamine oxidase.

3] 39 MECHANISM-BASED IRREVERSIBLE INHIBITORS R ~NH__NH2 *~,/°~NH2 Alkyl hydrazide Tranylcypromine It has been shown that N-nitrosolactams can inhibit chymotrypsin irreversibly, possibly by a mechanism that involves the formation of a carbonium ion [Eq. (10)]. 0 ,J CH30~ ° / / ' ° ~ 0 / 0 ~ 0 / / O t II I CH30/~,/'~,/N-N--O O ~ o / / ' ° ~ o / ° ~ O --Enz: I II O/~,/*'~CH ~ J Enz--OH (10) CH30~ e / / . /* ~/O Enz Enz--OH = chymotrypsin Interestingly, the open-chain analog of the above inhibitor is a substrate for the enzyme but not an inhibitor; this underlines the importance of holding the inhibitor at the active site to ensure enough time for chemical reaction.

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Affinity labeling by Nathan P. Kaplan, Nathan P. Colowick, William B. Jakoby, Meir Wilchek


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